Lysergamine (M3.2) Aptamer Details
ID# 7835
Structure
Chemistry
DNA
Target
Lysergamine, metergoline, and other small ergot alkaloids
Category
Protein
Affinity (Kd)
44 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCp dApdGpdCp dApdCpdAp dGpdApdGp dGpdTpdCp dApdGpdAp dTpdGpdGp dCpdGpdTp dCpdApdGp dCpdCpdCp dCpdGpdAp dTpdCpdGp dCpdCpdAp dTpdCpdCp dApdGpdGp dGpdApdCp dTpdCpdCp dCpdCpdCp dCpdTpdAp dCpdTpdGp dCpdCp dTpdApdTp dGpdCpdGp dTpdGpdCp dTpdApdCp dCpdGpdTp dGpdApdAp-3'
Molecular Weight: 24516.84
Extinction Coefficient: 747200
Binding Conditions / Buffer
Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 5 mM KCl, 2 mM MgCl2 ?ú6 H2O, 1 mM CaCl2?ú2 H2O.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
ER Martin et al. (2012) Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. Int. J. Mol. Sci. 13: 17138-17159.
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