Lup an 1 allergen β-conglutin (SGQ) Aptamer Details
ID# 7785
Structure
Chemistry
DNA
Target
β-conglutin
Category
Protein
Affinity (Kd)
0.58 nM
Length
11
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGp dGpdTpdGp dGpdGpdGp dGpdTpdGp dGp-3'
Molecular Weight: 3509.33
Extinction Coefficient: 109700
Binding Conditions / Buffer
Binding Buffer: 10 mM phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
P Polo. Selection, characterisation and analytical application of DNA aptamer against the anaphylactic toxic allergen, β-conglutin, Lup an 1. (Doctoral Thesis). Retrieved from Tesis Doctorals en Xarxa. http://hdl.handle.net/10803/84036.
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