Lup an 1 allergen, ??-Conglutin (#40) Aptamer Details
ID# 7784
Structure
Chemistry
DNA
Target
??-Conglutin
Category
Protein
Affinity (Kd)
360 nM
Length
93
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCp dTpdGpdAp dCpdApdCp dApdGpdCp dApdGpdGp dTpdTpdGp dGpdTpdGp dGpdGpdGp dGpdTpdGp dGpdCpdTp dTpdCpdCp dApdGpdTp dTpdGpdGp dGpdTpdTp dGpdApdCp dApdApdTp dApdCpdGp dTpdApdGp dGpdGpdAp dCpdApdCp dGpdApdAp dGpdTpdCp dCpdApdAp dCpdCpdAp dCpdGpdAp dGpdTpdCp dGpdApdGp dCpdApdAp dTpdCpdTp dCpdGpdAp dApdApdTp -3'
Molecular Weight: 28903.79
Extinction Coefficient: 915500
Binding Conditions / Buffer
Binding Buffer: 10 mM Phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4
Binding Temp: 21°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
P Nadal et al. DNA aptamers against the Lup an 1 food allergen. PLoS One 7(2012):e35253.
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