LM3 Aptamer Details
ID# 8209
Structure
Chemistry
DNA
Target
Listeria monocytogenes
Category
Cells
Affinity (Kd)
8.89 nM
Length
50
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdGpdTpdCpdCpdCpdGpdGpdTpdApdGpdTpdCpdTpdGpdApdGpdCpdTpdTpdApdCpdApdApdTpdGpdTpdCpdTpdGpdApdTpdTpdGpdGpdApdApdApdTpdGpdCpdApdCpdApdCpdCpdApdApdGp-3'
Molecular Weight: 15449
Extinction Coefficient:
Binding Conditions / Buffer
1X PBS buffer containing 100 mM, NaCl, 5 mM MgCl2, 2.5 mM KCl, and 0.005% Tween-20
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Deniz Yılmaz, Tuğdem Muslu, Ayhan Parlar, Hasan Kurt, Meral Yüce, SELEX against whole-cell bacteria resulted in lipopolysaccharide binding aptamers, Journal of Biotechnology, Volume 354, 2022, Pages 10-20, ISSN 0168-1656.
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