LipL32 DNA Aptamer LepDapt-5a Aptamer Details
ID# 8409
Structure
Chemistry
DNA
Target
LipL32 Protein
Category
Protein
Affinity (Kd)
33.97 nM
Length
28 bp
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdApdGpdCpdApdGpdGpdGpdGpdCpdGpdGpdGpdTpdGpdGpdGpdTpdGpdGpdGpdTpdGpdCpdTpdGpdCp-3'
Molecular Weight: 18.48 kDa
Extinction Coefficient:
Binding Conditions / Buffer
10 mM HEPES-KOH [pH 7.4], 150 mM NaCl
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yeoh TS, Tang TH, Citartan M. Isolation of a novel DNA aptamer against LipL32 as a potential diagnostic agent for the detection of pathogenic Leptospira. Biotechnol J. 2023 Mar;18(3):e2200418. doi: 10.1002/biot.202200418. Epub 2022 Dec 8. PMID: 36426669.
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