Aptagen

Leucomalachite green and Malachite green (A3-a) Aptamer Details

ID# 10044
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Structure
Leucomalachite green and Malachite green (A3-a) structure
Chemistry
DNA
Target
Leucomalachite green (LMG) and malachite green (MG)
Category
Small Organic
Affinity (Kd)
8.2 ± 1.2 nM (LMG) and 2.3 ± 0.2 μM (MG) nM
Length
45
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dCpdApdCpdTpdCpdCpdApdCpdGpdCpdApdTpdApdGpdGpdGpdApdCpdGpdCpdGpdApdApdTpdTpdGpdCpdGpdGpdApdCpdCpdTpdApdTpdGpdTpdGpdTpdGpdGpdTpdGpdTpdGp-3'
Molecular Weight: 13918 g/mole
Extinction Coefficient: 428800

Binding Conditions / Buffer

0.9 mM CaCl2, 2.68 mM KCl, 1.47 mM KH2PO4, 0.5 mM MgCl2·6H2O, 136.8 mM NaCl, 8.1 mM Na2HPO4·12H2O, pH 7.4
Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Wenwei Wu, Qifeng Sun, Tiansong Li, Kexin Liu, Yan Jiang, Yi Wang, Yongjie Yang, Selection and characterization of bispecific aptamers against malachite green and leucomalachite green, Analytical Biochemistry, Volume 658, 2022,114849, ISSN 0003-2697, https://doi.org/10.1016/j.ab.2022.114849.
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