LepDapt9149 Aptamer Details
ID# 9709
Structure
Chemistry
DNA
Target
Lipl32 protein
Category
Protein
Affinity (Kd)
64.59 (+/-) 11.25 from direct ELASA nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dCpdGpdGpdApdGpdApdGpdCpdCpdGpdApdGpdApdTpdTpdTpdApdGpdTpdGpdGpdCpdCpdGpdApdTpdGpdApdGpdGpdGpdGpdApdApdGpdApdApdApdCpdGpdTpdApdTpdTpdCpdCpdTpdTpdCpdGpdApdApdCpdGpdCpdCpdGpdTpdGpdCpdCpdGpdApdTpdApdTpdGpdCpdTpdCpdApdTpdCpdCpdApdCpdApdTpdGpdCp
Molecular Weight: 24754 g/mole
Extinction Coefficient: 774800
Binding Conditions / Buffer
A tripartite binding SELEX was chosen with different conditions for all the mechanisms. In the nitrocellulose filter membrane step and the microtiter plate-based partitioning, blockers were used during selection to prevent non-specific binding
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yeoh, T.S., Cheah, HL., Yoke, L.F. et al. Isolation of DNA aptamers by Tripartite-hybrid-high throughput SELEX (Tripartite-hybrid-HT SELEX) for the detection of pathogenic Leptospira. World J Microbiol Biotechnol 41, 454 (2025). https://doi.org/10.1007/s11274-025-04637-8
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