LepDapt-5561 Aptamer Details

ID# 9713

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Structure

Chemistry

DNA

Target

Lipl32 protein

Sequence (3-char table code)

G A C U/T

dCpdGpdGpdApdGpdApdGpdCpdCpdGpdApdGpdApdTpdTpdTpdApdGpdTpdGpdGpdGpdGpdGpdCpdApdGpdApdGpdGpdGpdGpdApdGpdApdTpdGpdGpdApdApdTpdApdGpdCpdGpdCpdGpdApdTpdGpdTpdGpdApdTpdGpdGpdGpdCpdCpdApdCpdGpdApdTpdApdTpdGpdCpdTpdCpdApdTpdCpdCpdApdCpdApdTpdGpdCp

Molecular Weight: 25004.2 g/mole

Extinction Coefficient: 794700

Binding Conditions / Buffer

Obtained in cycle 9. Tripartite partitioning mechanism used to develop the aptamer. Washing - 1X PBST Binding takes place in presence of blocking agents to prevent non-specific binding.

Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Yeoh, T.S., Cheah, HL., Yoke, L.F. et al. Isolation of DNA aptamers by Tripartite-hybrid-high throughput SELEX (Tripartite-hybrid-HT SELEX) for the detection of pathogenic Leptospira. World J Microbiol Biotechnol 41, 454 (2025). https://doi.org/10.1007/s11274-025-04637-8

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