Lead (Pb) binding aptamer Aptamer Details
ID# 7709
Structure
Chemistry
DNA
Target
Lead (Pb)
Category
Other
Affinity (Kd)
1 nM
Length
15
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGp-3'
Molecular Weight: 4726.13
Extinction Coefficient: 143300
Binding Conditions / Buffer
pH 7.0, 20 mM NaNO3, 8 mM Tris nitrate, 100 μM NaCN, 25 °C
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
B-F Ye et al. "Colorimetric photonic hydrogel aptasensor for the screening of heavy metal ions." Nanoscale 4(2012): 5998-6003.
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