LCW17 Aptamer Details
ID# 9553
Structure
Chemistry
DNA
Target
Vigilin Protein
Category
Protein
Affinity (Kd)
25 ± 3 nM
Length
58 nucleotides
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dApdGpdCpdGpdTpdCpdGpdApdApdTpdApdCpdCpdApdCpdTpdApdCpdApdGpdApdApdTpdApdCpdCpdCpdTpdApdApdApdApdCpdCpdTpdApdApdApdCpdTpdApdCpdCpdTpdApdTpdCpdApdApdCpdCpdTpdApdApdTpdGpdGpdApdGpdCpdTpdCpdGpdTpdGpdGpdTpdCpdApdGp
Molecular Weight: ≈ 21.4 kDa
Extinction Coefficient:
Binding Conditions / Buffer
Bound in DPBS with 5 mM MgCl₂, 1–2 % BSA, and 2 µg/µL yeast tRNA for 40 min at 4 °C or 1 h at room temperature.
Binding Temp: 25°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Liu, C., Jiang, W., Tian, X., Yang, P., Xiao, L., Li, J., Qiu, L., Tu, H., & Tan, W. (2019). Identification of vigilin as a potential ischemia biomarker by brain slice-based systematic evolution of ligands by exponential enrichment. Analytical Chemistry, 91(11), 6675–6681. https://doi.org/10.1021/acs.analchem.9b00609
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