Aptagen

Lactobacillum acidophilius (hemag1P) Aptamer Details

ID# 7607
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Structure
Lactobacillum acidophilius (hemag1P) structure
Chemistry
DNA
Target
Lactobacillum acidophilius
Category
Cells
Affinity (Kd)
13 nM
Length
78
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdTpdApdGpdCpdCpdCpdTpdTpdCpdApdApdCpdApdTpdApdGpdTpdApdApdTpdApdTpdCpdTpdCpdTpdGpdCpdApdTpdTpdCpdTpdGpdTpdGpdTpdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
Molecular Weight: 24010.64
Extinction Coefficient: 751500

Binding Conditions / Buffer

Washed twice with binding buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2
Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Hamula et al. "Selection of Aptamers against Live Bacterial Cells." Analytical Chemistry, 80(2008): 7812-7819.
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