L1 Aptamer Details
ID# 9678
Structure
Chemistry
DNA
Target
IGFBP3 (Insulin-like Growth Factor Binding Protein 3)
Category
Protein
Affinity (Kd)
8 nM
Length
53
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdGpdGpdApdTpdGpdTpdCpdApdGpdTpdCpdCpdGpdGpdCpdApdCpdGpdCpdApdTpdTpdApdCpdCpdCpdCpdTpdGpdTpdTpdCpdCpdCpdCpdCpdTpdTpdGpdTpdTpdGpdTpdGpdApdCpdApdCpdApdTpdCpdCp-3'
Molecular Weight: 16122.4 g/mole
Extinction Coefficient: 475500
Binding Conditions / Buffer
Library was injected into ovarian bursa of female C57BL6/athymic nude mice for in vivo SELEX.
4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL BSA, 0.1 mg/mL salmon sperm DNA in DPBS, pH 7.4 at 37 °C for 30 min in the dark.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Li, C., Chen, W., Zhang, Z., Qiu, F., Jiang, R., Fang, X., Zhang, S., Li, L., Liao, G., Shen, Y., Tang, H., Meng, H., Zhu, T., Luo, Z., & Tan, W. (2025). In Vivo SELEX: A Strategy for Identifying Ovarian Cancer-Specific Aptamers. ACS applied materials & interfaces, 17(46), 63162–63171. https://doi.org/10.1021/acsami.5c17714
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