L-vasopressin Aptamer Details
ID# 7820
Structure
Chemistry
L-DNA
Target
L-vasopressin/arginine vasopressin/AVP/ADH
Category
Peptide
Affinity (Kd)
1.2 µM
Length
55
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-LdTLdCLdA LdCLdGLdT LdGLdCLdA LdTLdGLdA LdTLdALdG LdALdCLdG LdGLdCLdG LdALdALdG LdCLdCLdG LdTLdCLdG LdALdGLdT LdTLdGLdC LdTLdGLdT LdGLdTLdG LdCLdCLdG LdALdTLdG LdCLdALdC LdGLdTLdG LdA-3'
Molecular Weight: 44304.78
Extinction Coefficient: 527800
Binding Conditions / Buffer
Buffer B: 20 mM sodium 2-[bis(2-hydroxyethyl)amino]ethane sulfonate, pH 7.3, with 140 mM NaCl, 5 mM KCl, 1.5 mM MgCl2, 1 mM CaCl2, and 0.02% Triton X-100.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
K. P. Williams, D. P. Bartel, et al. Bioactive and nuclease-resistant l-DNA ligand of vasopressin. Proc. Natl. Acad. Sci., USA. 94(1997): 11285-11290.
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