L-tryptophan(Trp 70-727) Aptamer Details
ID# 7590
Structure
Chemistry
RNA
Target
L-tryptophan
Category
Protein
Affinity (Kd)
15 nM
Length
104
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprUprCprCprUprAprAprGprCprUprCprUprAprUprCprGprGprCprUprGprGprAprCprGprAprCprGprGprGprGprAprCprGprCprCprAprCprUprGprGprAprCprUprAprGprGprUprAprAprGprCprCprAprGprGprAprCprCprGprUprAprCprGprUprCprGprGprGprAprGprCprCprGprUprCprAprGprAprAprUprAprAprAprAprGprCprGprGprCprCprUprAprGprCprGprAprUprCprGprAprUp-3'
Molecular Weight: 33718.43
Extinction Coefficient: 1026700
Binding Conditions / Buffer
Selection buffer: 50 mM HEPES (pH 7.0), 250 mM NaCl, 5 mM MgCl2, 5 mM CaCl2, 5 mM glycine
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Majerfeld and Yarus. "A diminutive and specific RNA binding site for L-tryptophan." Nucleic Acids Research, 33(2005): 5482-5493.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
