L-Selectin (LD201t1) Aptamer Details
ID# 7772
Structure
Chemistry
DNA
Target
L-Selectin binding to sialyl Lewis X
Category
Protein
Affinity (Kd)
3 nM
Length
49
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdApdGpdCpdCpdApdApdGpdGpdTpdApdApdCpdCpdApdGpdTpdApdCpdApdApdGpdGpdTpdGpdCpdTpdApdApdApdCpdGpdTpdApdApdTpdGpdGpdCpdTpdTpdCpdGpdGpdCpdTpdTpdApdCp-3'
Molecular Weight: 15113.89
Extinction Coefficient: 481400
Binding Conditions / Buffer
Binding Buffer (20 mM Hepes, 111 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl, 8.9 mM NaOH final pH 8) and 1% globulin-free BSA.
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hicke, Brain, et al. "DNA Aptamers Block L-Selectin Function In Vivo Inhibition of Human Lymphocyte Traf?cking in SCID Mic." Journal of Clinical Investigation, 98 (1996): 2688-2692
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