L-Selectin (LD201) Aptamer Details
ID# 7466
Structure
Chemistry
DNA
Target
L-Selectin
Category
Protein
Affinity (Kd)
1.8 nM
Length
79
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdTpdApdCpdCpdTpdApdCpdGpdApdTpdCpdTpdGpdApdCpdTpdApdGpdCpdCpdApdApdGpdGpdTpdApdApdCpdCpdApdGpdTpdApdCpdApdApdGpdGpdTpdGpdCpdTpdApdApdApdCpdGpdTpdApdApdTpdGpdGpdCpdTpdTpdCpdGpdGpdCpdTpdTpdApdCpdTpdCpdTpdCpdApdTpdGpdTpdApdGpdTpdTpdCpdCp-3'
Molecular Weight: 24243.79
Extinction Coefficient: 754700
Binding Conditions / Buffer
Binding Buffer (20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl) plus 0.01% (wt/vol) human serum albumin.
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hicke, Brain, et al. "DNA Aptamers Block L-Selectin Function In Vivo Inhibition of Human Lymphocyte Traf?cking in SCID Mic." Journal of Clinical Investigation, 98 (1996): 2688-2692.
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