L-Isoleucine (IL 42-32b) Aptamer Details
ID# 7518
Structure
Chemistry
RNA
Target
L-Isoleucine
Category
Small Organic
Affinity (Kd)
200 µM
Length
32
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-P-rGprGprUprCprUprUprAprCprGprUprCprGprUprUprCprGprCprGprAprCprUprAprUprUprGprGprGprAprGprAprCprCp-3'
Molecular Weight: 10233.16
Extinction Coefficient: 310800
Binding Conditions / Buffer
Selection Buffer (10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl) Reaction Buffer (50 mM sodium borate (pH 8.0), 42 mM KCl, 7.5 mM MgCl2, and 0.4 mM ZnCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Majerfeld and Yarus. "Isoleucine:RNA sites with associated coding sequence." RNA, 4 (1998): 471-478.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
