L-Histidine (His 945) Aptamer Details
ID# 7520
Structure
Chemistry
RNA
Target
L-Histidine
Category
Small Organic
Affinity (Kd)
12 µM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprCprAprUprCprGprGprAprAprAprGprUprGprGprGprUprUprGprAprUprGprUprAprAprGprUprAprAprCprAprGprGprCprGprAprUprGprCprCp-3'
Molecular Weight: 13017.94
Extinction Coefficient: 410400
Binding Conditions / Buffer
Selection Buffer (50 mM Hepes (pH 7.0), 250 mM NaCl, 5 mM each CaCl2 and MgCl2, and 1 mM glycine)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Majerfeld, et al. "RNA Affnity for Molecular L-Histidine; Genetic Code Origins." Journal of Molecular Evolution, 61 (2005): 226-235.
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