Aptagen

L-Arginine (ag.06) Aptamer Details

ID# 7460
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Structure
L-Arginine (ag.06) structure
Chemistry
RNA
Target
L-Arginine
Category
Small Organic
Affinity (Kd)
330 nM
Length
97
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-rGprGprAprGprCprUprCprAprGprCprCprUprUprCprAprCprUprGprCprAprUprGprAprUprAprAprAprCprCprGprAprUprGprCprUprGprGprGprCprGprAprUprUprCprUprCprCprUprGprAprAprGprUprAprGprGprGprGprAprAprGprAprGprUprUprGprUprCprAprUprGprUprAprUprGprGprGprGprGprCprAprCprCprAprCprGprGprUprCprGprGprAprUprCprCprUprGp-3'
Molecular Weight: 31347.87
Extinction Coefficient: 952800

Binding Conditions / Buffer

Binding buffer: 250 mM NaCl, 50 mM Trisƒ??HCl (pH 7.6), 5 mM MgCl2
Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Geiger, Albert, et al. "RNA aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivity." Nucleic Acid Research, 24 (1996): 1029-1036.
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