Aptagen

L-Apt1-12 Aptamer Details

ID# 9737
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Structure
L-Apt1-12 structure
Chemistry
L-RNA
Target
EBNA1 D-rG4
Category
Other
Affinity (Kd)
105.6±30.6 nM
Length
25
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-LrGLrCLrCLrCLrULrALrULrULrGLrGLrALrGLrGLrCLrULrULrGLrGLrGLrCLrALrGLrGLrGLrC-3'
Molecular Weight: 7684.9 g/mole
Extinction Coefficient: 225600

Binding Conditions / Buffer

25 mM Tris-HCl (pH 7.5), 1 mM MgCl2, 150 mM KCl
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

D. Ji, B. Wang, K. W. Lo, C. M. Tsang, C. K. Kwok, Angew. Chem. Int. Ed. 2025, 64, e202417247. https://doi.org/10.1002/anie.202417247
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