L-Apt.8f Aptamer Details
ID# 9767
Structure
Chemistry
L-RNA
Target
APP D-rG4
Category
Other
Affinity (Kd)
24.06 ± 2.90 nM
Length
33
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-LrGLrCLrGLrGLrCLrALrALrGLrALrGLrULrGLrULrGLrGLrGLrALrGLrGLrGLrGLrGLrGLrULrCLrGLrALrCLrGLrCLrCLrGLrC-3'
Molecular Weight: 10324.6 g/mole
Extinction Coefficient: 317500
Binding Conditions / Buffer
25 mM Tris-HCl (pH 8.0), 1 mM MgCl2, 150 mM KCl
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Novel L-RNA Aptamer Controls APP Gene Expression in Cells by Targeting RNA G-Quadruplex Structure
Haizhou Zhao, Hei Yuen Wong, Danyang Ji, Kaixin Lyu, and Chun Kit Kwok
ACS Applied Materials & Interfaces 2022 14 (27), 30582-30594
DOI: 10.1021/acsami.2c06390
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