Aptagen

Kit-129 Aptamer Details

ID# 7866
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Structure
Kit-129 structure
Chemistry
DNA
Target
CD117 (c-kit)
Category
Protein
Affinity (Kd)
12.21 nM
Length
29
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdCpdTpdCpdApdApdCpdGpdCpdGpdGpdGpdApdCpdGpdGpdCpdTpdCpdTpdCpdCpdCpdApdTpdTpdGpdApdCp-3'
Molecular Weight: 8839.75
Extinction Coefficient: 262400

Binding Conditions / Buffer

buffer composed of Dulbeccoƒ??s phosphate-buffered saline with calcium and magnesium and 5 mM MgCl2, 0.45% glucose and 100 mg/ml tRNA. 1 hr incubation with cells
Binding Temp: 4°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents Susanne Meyer et al. 2013
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