Keratinocyte Growth Factor (14F) Aptamer Details

ID# 7542

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Structure

Chemistry

2'-F-RNA

Target

Keratinocyte Growth Factor (KGF)

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGpfUprGprGpfUpfCpfUpfCpfCpfCprAprApfUpfUpfCpfUprAprAprApfCpfUpfUpfUpfCpfUpfCpfCprApfUpfCprGpfUprApfUpfCpfUprGprGprGpfCprAprGprApfCprGprApfCpfUpfCprGpfCpfCpfCprGprAp-3'

Molecular Weight: 22180.13

Extinction Coefficient: 664400

Binding Conditions / Buffer

Binding Buffer (DPBS, 0.5 mM MgCl2, 0.2% BSA, 0.02% sodium azide for PC-3, serum-free MEM growth medium, 0.1% BSA, 25 mM HEPES (pH 7.4), 1 ug/ml heparin for NIH3T3/KGFR)

Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Pagratis, N., et al. "Potent 2'-amino-, and 2'-fluoro-2'- deoxyribonucleotide RNA inhibitors of keratinocyte growth factor." Nature Biotechnology, 25(1997):68-73.

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