Kanamycin B (K8) Aptamer Details

ID# 7523

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Structure

Chemistry

RNA

Target

Kanamycin B

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprCprUprCprGprGprUprAprCprCprGprAprAprUprUprCprUprCprGprCprCprCprUprAprUprAprGprGprGprGprUprGprUprUprGprAprGprGprGprAprAprAprUprGprUprGprUprGprCprGprAprCprAprAprGprGprUprGprCprGprGprUprGprGprCprCprAprGprAprAprCprUprUprUprUprCprGprUprUprCprUprCprAprUprCprAprAprAprAprGprCprUprUprUprGprCprAprGprAprGprGprAprUprCprCprUprUp-3'

Molecular Weight: 35819.5

Extinction Coefficient: 1095000

Binding Conditions / Buffer

Binding Buffer: 140 mM NaCl, 5 mM KCl, lmM MgCl2, and 20 mM HEPES at pH 7.5

Binding Temp: 20°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Kwon et al., "In Vitro Selection of RNA against Kanamycin B." Molecules and Cells, 11 (2001): 303-311.

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