Kanamycin aptamer Aptamer Details
ID# 9817
Structure
Chemistry
DNA
Target
Kanamycin
Category
Small Organic
Affinity (Kd)
51 nM
Length
42
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdApdCpdGpdApdCpdGpdCpdApdGpdTpdCpdGpdGpdCpdTpdTpdTpdTpdGpdCpdGpdGpdGpdApdApdApdApdApdGpdGpdTpdCpdGpdGpdApdGpdTpdCpdGpdTpdCp-3'
Molecular Weight: 13060.5 g/mole
Extinction Coefficient: 407300
Binding Conditions / Buffer
Binding buffer - (20 mM HEPES, pH 7.5, 150 mM NaCl, 2 mM MgCl2)
High-affinity aptamers may fail to enrich if their hybridization to the capture strand is insufficiently stable under the selection condition. The authors suggest that a high salt condition could help and that they used 500 mM NaCl and 10 mM MgCl2 helped in raising the Tm enough that it prevented detachment from the capture strand on the beads.
Binding Temp:
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
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