Kanamycin A (sla 16) Aptamer Details
ID# 7555
Structure
Chemistry
RNA
Target
Kanamycin A
Category
Small Organic
Affinity (Kd)
300 nM
Length
80
Aptazyme
Yes
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprUprUprCprAprCprCprGprCprGprGprGprGprUprUprGprCprGprGprAprCprCprGprGprGprAprGprCprUprCprCprAprGprCprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUp-3'
Molecular Weight: 25761.54
Extinction Coefficient: 773600
Binding Conditions / Buffer
Binding Buffer (500 mM NaCl, 50 mM Tris (pH 7.6))
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lato et al. "In vitro selection of RNA lectins: using combinatorial chemistry to interpret ribozyme evolution." Chemistry & Biology, 2(1995): 291-303.
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