Aptagen

K16-1 Aptamer Details

ID# 8148
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Structure
K16-1 structure
Chemistry
DNA
Target
Kanamycin A
Category
Small Organic
Affinity (Kd)
342±74 nM
Length
270
Aptazyme
No

Sequence (3-char table code)

G A C U/T
dCpdGpdCpdApdTpdApdCpdCpdApdGpdCpdTpdTpdApdGpdTpdTpdCpdApdGpdApdApdTpdTpdCpdApdTpdTpdGpdGpdApdGpdCpdGpdTpdGpdGpdCpdGpdTpdGpdGpdApdTpdGpdCpdCpdCpdGpdApdTpdGpdGpdApdCpdCpdGpdCpdCpdCpdCpdApdGpdGpdGpdTpdGpdCpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTpdCpdGpdGpdCp
Molecular Weight: 28183.1
Extinction Coefficient: 861900

Binding Conditions / Buffer

50% 100 mM sodium chloride, 20 mM tris-hydrochloride, 2 mM magnesium chloride, 5 mM potassium chloride, and 1 mM calcium chloride (pH 7.6); 50% 1X Cut Smart Buffer (New England Biolabs) 50 mM potassium acetate, 20 mM tris-acetate, 10 mM magnesium acetate, 100 µg/mL bovine serum albumin (pH 7.9)
Binding Temp: 20°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

(This content is a preprint and has not been peer-reviewed.)
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