JH19 Aptamer Details
ID# 7880
Structure
Chemistry
DNA
Target
NB4 AML cell line
Category
Cells
Affinity (Kd)
7.57 nM
Length
89
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdApdCpdGpdCpdTpdTpdApdCpdTpdCpdApdGpdGpdTpdGpdTpdGpdApdCpdTpdCpdGpdApdGpdGpdTpdGpdTpdGpdApdCpdTpdCpdGpdApdTpdCpdTpdGpdTpdGpdGpdGpdGpdGpdTpdTpdGpdGpdGpdGpdGpdGpdTpdGpdGpdTpdTpdTpdTpdTpdCpdGpdGpdApdApdCpdGpdApdApdGpdGpdApdCpdGpdCpdApdGpdApdTpdGpdApdApdGpdTpdCpdTpdCp
Molecular Weight: 27806.05
Extinction Coefficient: 858700
Binding Conditions / Buffer
4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS)
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yang, M. et al. "Developing aptamer probes for acute myelogenous leukemia detection and surface protein biomarker discovery." Journal of Hematology & Oncology 2014, 7:5.
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