J7 Aptamer Details

ID# 9000

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Structure

Chemistry

DNA

Target

CD3ε

Sequence (3-char table code)

G A C U/T

dApdApdGpdGpdApdGpdCpdApdGpdCpdGpdTpdGpdGpdApdGpdGpdApdTpdApdTpdCpdGpdGpdTpdApdApdGpdGpdGpdTpdCpdGpdGpdGpdGpdApdTpdGpdCpdTpdApdCpdApdApdCpdTpdGpdTpdTpdTpdApdApdApdCpdGpdApdCpdCpdCpdGpdTpdCpdCpdApdTpdTpdApdGpdGpdGpdTpdGpdTpdGpdTpdCpdGpdTpdCpdGpdTpdGpdGpdTp

Molecular Weight: 26516.1 g/mole

Extinction Coefficient: 837200

Binding Conditions / Buffer

incubating 10.8 nmol denatured HPLC (high-performance liquid chromatography)-purified single-stranded DNA (ssDNA) library with 1.0 × 107 cells in a final volume of 500 μL.

Binding Temp: The DNA library suspended in 250 μL RPMI was denatured by heating at 95°C for 5 min, followed by folding for 45 min at 25°C in order to allow the formation of proper secondary structures.

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Zumrut HE, Batool S, Argyropoulos KV, Williams N, Azad R, Mallikaratchy PR. Integrating Ligand-Receptor Interactions and In Vitro Evolution for Streamlined Discovery of Artificial Nucleic Acid Ligands. Mol Ther Nucleic Acids. 2019 Sep 6;17:150-163. doi: 10.1016/j.omtn.2019.05.015. Epub 2019 Jun 4. PMID: 31255977; PMCID: PMC6606840.

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