iSN04 Nucleolin Aptamer Aptamer Details
ID# 8957
Structure
Chemistry
DNA
Target
Nucleolin
Category
Protein
Affinity (Kd)
Internalized to cells grown in DMEM, 15% FBS, 1% non-essential amino acids (NEAA) (Fujifilm Wako Chemicals), 1% nucleosides (Merck, Darmstadt, Germany), 0.1 mM 2-mercaptoethanol (Nacalai), 103 units/mL LIF (Nacalai), 3 uM CHIR99021 (FujifilmWako Chemicals), 0.8 uM PD0325901 (FujifilmWako Chemicals), and P/S
Length
18
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dAsdGsdAsdTsdTsdAsdGsdGsdGsdTsdGsdAsdGsdGsdGsdTsdGsdAs-3'
Molecular Weight: 5956.8
Extinction Coefficient: 193500
Binding Conditions / Buffer
37°C
Binding Temp:
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ishioka M, Nihashi Y, Sunagawa Y, et al. Myogenetic Oligodeoxynucleotide Induces Myocardial Differentiation of Murine Pluripotent Stem Cells. Int J Mol Sci. 2023;24(18):14380. Published 2023 Sep 21. doi:10.3390/ijms241814380
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