IR-A43 Aptamer Details
ID# 8101
Structure
Chemistry
DNA
Target
recombinant insulin receptor extracellular domain
Category
Protein
Affinity (Kd)
70 nM
Length
81
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdApdTpdGpdApdGpdTpdGpdApdCpdCpdGpdTpdCpdCpdGpdCpdCpdTpdGpdTsdApdTsdCpdCpdGpdCpdApdGpdTsdApdTsdCpdGpdGpdCpdApdTsdTsdCpdApdGpdCpdGpdApdCpdCpdCpdGpdGpdGpdCpdCpdApdCpdGpdApdCpdNpdApdApdCpdApdGpdCpdCpdApdCpdApdCpdCpdApdCpdCpdApdGpdCpdCpdApdApdAp-3’
Molecular Weight: 22946.6
Extinction Coefficient: 711800
Binding Conditions / Buffer
40 mM HEPES (pH 7.5), 102 mM NaCl, 5 mM KCl, 5 mM MgCl2, and
0.05% Tween-20
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yunn NO, Park M, Park S, Lee J, Noh J, Shin E, Ryu SH. A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer. Nucleic Acids Res. 2021 Jan 25;49(2):700-712. doi: 10.1093/nar/gkaa1247. PMID: 33410883; PMCID: PMC7826266.
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