Internalin A of Listeria monocytogenes Aptamer Details
ID# 7769
Structure
Chemistry
DNA
Target
Internalin A (InlA)
Category
Protein
Affinity (Kd)
103 CFU/mL
Length
47
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dApdTpdCpdCpdApdTpdGpdGpdGpdGp dCpdGpdGpdApdGpdApdTpdGpdApdGp dGpdGpdGpdGpdApdGpdGpdApdGpdGp dGpdCpdGpdGpdGpdTpdApdCpdCpdCp dGpdGpdTpdTpdGpdApdTp-3'
Molecular Weight: 14811.63
Extinction Coefficient: 470400
Binding Conditions / Buffer
TBS: Tris 10 mM, NaCl 0.85%, pH 8.0
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
S.H. Ohk et al. Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food. J. Appl. Microbiol. 109(2010): 808-817.
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