Interleukin 32 (IL-32) (AC3-3) Aptamer Details
ID# 7524
Structure
Chemistry
RNA
Target
Interleukin-32 (IL-32)
Category
Protein
Affinity (Kd)
78 nM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUprCprCprGprGprAprGprAprGprAprAprGprGprGprUprCprAprAprAprGprUprUprGprUprGprCprGprGprGprAprGprUprGprUprGprUprUprGprUprGprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprUprUprCp-3'
Molecular Weight: 29155.58
Extinction Coefficient: 905300
Binding Conditions / Buffer
Binding Buffer (20 mM sodium phosphate, 500 mM NaCl and 20 mM imidazole (pH 7.4))
Elution Buffer (20 mM sodium phosphate, 500 mM NaCl and 300 mM imidazole (pH 7.4)
At room temperature.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kim et al. "Generation of Antagonistic RNA Aptamers Specific to Proinflammatory Cytokine Interleukin-32." Bulletin of the Korean Chemical Society, 31 (2010): 3561-3566.
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