Interferon-Gamma Aptamer Details

ID# 7971

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Structure

Chemistry

DNA

Target

Interferon-gamma (IFN-c)

Sequence (3-char table code)

G A C U/T

5'-dCpdCpdGpdCpdCpdCpdApdApdApdTpdCpdCpdCpdTpdApdApdGpdApdGpdApdApdGpdApdCpdTpdGpdTpdApdApdTpdGpdApdCpdApdTpdCpdApdApdApdCpdCpdApdGpdApdCpdApdCpdApdCpdTpdApdCpdApdCpdApdCpdGpdCpdAp-3'

Molecular Weight: 18001.76

Extinction Coefficient: 585100

Binding Conditions / Buffer

Salmon sperm DNA (0.1 mg/mL) and BSA (1 mg/mL) were added to the binding buffer (PBS) to reduce the background interference

Binding Temp: The procedures of selection were as follows: in the first selection round, the ssDNA pool (200 pmol) was first heated at 95uC for 5 min and then cooled to 0uC for 15 min immediately.

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Cao, B., Hu, Y., Duan, J., Ma, J., Xu, D., & Yang, X. D. (2014). Selection of a novel DNA aptamer for assay of intracellular interferon-gamma. PloS one, 9(5), e98214.

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