Interferon-? (2’NH2-30) Aptamer Details
ID# 7469
Structure
Chemistry
DNA
Target
Interferon-y/IFN-y/IFNG
Category
Protein
Affinity (Kd)
2.7 nM
Length
70
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprApnCprGprApnUprGpnCprGprGp nCprAprGprGpnUprAprApnUpnUprApnCprApnUprGprAprAprGprGpnUprGprGprGpnUpnUprAprGprGpnUprApnCpnUpnUpnUpnCprAprGprGprGpnUp nCprAprGprApnCprGprApnCpnUpnCprGpnCpnCpnCprGprAp-3'
Molecular Weight: 38579.5
Extinction Coefficient: 710100
Binding Conditions / Buffer
Binding Mixture: PBS (138 mM NaC1, 2.7 mM KCl, 8.1 mM Na2HPO4, and 1.1 mM KH2PO4, pH 7.4) containing 1 mM Mg2+ ions and 0.01% human serum albumin.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kubik, Mark F., et al. "Isolation and Characterization of 2ƒ??-Fluoro-, 2ƒ??-Amino-, and 2ƒ??-Fluoro-/Amino-Modified RNA Ligands to Human IFN-7 That Inhibit Receptor Binding." Journal of Immunology, 159 (1997): 259-267.
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