Integrin ??3 subunit (??3-1) Aptamer Details

ID# 7705

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Structure

Chemistry

2'-F-RNA

Target

Integrin ??3 subunit

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGpfCpfCpfCprAprGprApfUpfUprApfCpfUprGpfUprGprGprAprGpfUprGprGpfUpfUprGpfUpfCpfUprGpfCprGprAprApfUpfCpfCpfUpfUpfCprGpfUpfCpfCprApfCpfCpfCprAprApfUprAprGpfCprAprGprApfCprGprApfCpfUpfCprGpfCpfCpfCprGprAp-3'

Molecular Weight: 26160.56

Extinction Coefficient: 784800

Binding Conditions / Buffer

Binding Buffer: PBS supplemented with 0.05% Tween-20 and 0.1% BSA. Incubation Time: 1 h. Folding Protocol: Heated to 95°C for 5 min and then snap-colled on ice of 5 min. Assay Type: ELONA

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Gong, Q. et al,(2012). Selection strategy to generate aptamer pairs that bind to distinct sites on protein targets. Analytical Chemistry, 2012(84), 5365-5371.

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