Insulin Receptor Antibody (MA20) (#1) Aptamer Details
ID# 7474
Structure
Chemistry
DNA
Target
Insulin Receptor Antibody (MA20)
Category
Antibody
Affinity (Kd)
30 nM
Length
86
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprGpnCprGprGprAprAprGpnCprGpnUprGpnCpnUprGprGprGpnCpnCpnUprGprGpnUpnUprGpnCpnUprGpnUprAprAprAprAprApnUprAprGprApnCprApnCprGprAprApnUpnCpnUprGpnCpnCprGprApnCpnCprApnUprAprApnCpnCpnCprAprGprAprGprGpnUpnCprGprApnUprGprGprApnUpnCpnCpnCpnCpnCpnCp-3'
Molecular Weight: 50020.06
Extinction Coefficient: 843600
Binding Conditions / Buffer
Binding buffer: 30 mM Tris-HCl (pH 7.5), 150 mM NaC1, 10 mM MgC12, 2 mM dithiothreitol, and 1.0% BSA
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lee, Seong-Wook and Sullenger, Bruce A. "Isolation of a Nuclease-resistant Decoy RNA That Selectively Blocks Autoantibody Binding to Insulin Receptors on Human Lymphocytes." Journal of Experimental Medicine, 184 (1996): 315-324.
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