Insulin-Linked Polymorphic Region Two-Repeat (ILPR2) Aptamer Details
ID# 7863
Structure
Chemistry
DNA
Target
Insulin
Category
Peptide
Affinity (Kd)
N/A nM
Length
28
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdCpdApdGpdGpdGpdGpdTpdGpdTpdGpdGpdGpdGpdApdCpdApdGpdGpdGpdGpdTpdGpdTpdGpdGpdGpdGp-3'
Molecular Weight: 8911.82
Extinction Coefficient: 283900
Binding Conditions / Buffer
Tris buffer (20 mM Tris, 5mM KCL, pH 7.3)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Connor A C, McGown L B, et al. (2006) Insulin Capture by an Insulin-Linked Polymorphic Region G-Quadruplex DNA Oligonucleotide. J Am Chem Soc. 128: 4986-4991.
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