Aptagen

Insulin Binding Aptamer (IBA) Aptamer Details

ID# 7960
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Structure
Insulin Binding Aptamer (IBA) structure
Chemistry
DNA
Target
Insulin
Category
Protein
Affinity (Kd)
n/a nM
Length
30
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdGpdTpdGpdGpdTpdGpdGpdGpdGpdGpdGpdGpdGpdTpdTpdGpdGpdTpdApdGpdGpdGpdTpdGpdTpdCpdTpdTpdCp-3'
Molecular Weight: 9493.19
Extinction Coefficient: 288200

Binding Conditions / Buffer

50 mM Tris-HCl, 10 mM KCl, 100 mM NaCl
Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Pu, Y.; Zhu, Z.; Han, D.; Liu, H.; Liu, J.; Liao, J.; Zhang, K.; & Tan, W. Insulin-Binding Aptamer-Conjugated Graphene Oxide for Insulin Detection. Analyst, 2011, 136, 4138-4140
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