IgE (4.4.12) Aptamer Details
ID# 7467
Structure
Chemistry
DNA
Target
IgE
Category
Protein
Affinity (Kd)
23 nM
Length
78
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdTpdGpdApdApdApdCpdApdTpdApdGpdCpdApdTpdApdTpdTpdTpdApdCpdTpdTpdApdTpdGpdTpdCpdGpdCpdCpdTpdTpdGpdCpdCpdGpdGpdTpdTpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
Molecular Weight: 24050.67
Extinction Coefficient: 753800
Binding Conditions / Buffer
Binding buffer: PBS and 1 mM MgCl2 (pH 8.0)
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mendonsa, Shaun D. and Bowser, Michael T. "In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis." Analytical Chemistry, 76 (2004): 5387-5392.
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