IFN-γ-binding aptamer Aptamer Details
ID# 8230
Structure
Chemistry
DNA
Target
IFN-γ
Category
Protein
Affinity (Kd)
3 nM
Length
34
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdGpdTpdTpdGpdGpdTpdTpdGpdTpdGpdTpdTpdGpdGpdGpdTpdGpdTpdTpdGpdTpdGpdTp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
The aptamer was dissolved in 10 mM HEPES buffer (pH 7.4 with 150 mM NaCl). This buffer was also employed in all IFN-γ sensor experiments.
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Development of an aptamer beacon for detection of interferon-gamma. Tuleuova, et. al (2010)
https://pubmed.ncbi.nlm.nih.gov/20121141/
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