IFNγ Aptamer Aptamer Details

ID# 9256

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Structure

Chemistry

DNA

Target

Interferon-gamma (IFNγ)

Sequence (3-char table code)

G A C U/T

dApdGpdGpdGpdGpdTpdTpdGpdGpdTpdTpdGpdTpdGpdTpdTpdGpdGpdGpdTpdGpdTpdTpdGpdTpdGpdTpdCpdCpdApdApdCpdCpdCpdCpdTp

Molecular Weight: Approx. 10,700 – 11,000 Da

Extinction Coefficient: 354

Binding Conditions / Buffer

The buffer is RPMI with 10% FBS, incubated at 37 °C (handled at room temperature). It uses 1 nmol per 10⁶ cells to detect IFNγ within 48 hours, anchored by 3′-CholTEG membrane insertion.

Binding Temp: Room temperature (22–25 )°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Luo, S., Notaro, A., & Lin, L. (2025). ATLAS-seq: a microfluidic single-cell TCR screen for antigen-reactive TCRs. Nature Communications, 16, 216. https://doi.org/10.1038/s41467-024-54675-3

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