i-6 Aptamer Details
ID# 8154
Structure
Chemistry
DNA
Target
Glycan modification
Category
Other
Affinity (Kd)
29.5 µM
Length
90
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
'5-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdTpdCpdTpdCpdGpdTpdGpdTpdGpdTpdTpdTpdGpdGpdTpdTpdTpdTpdApdGpdApdTpdTpdTpdTpdTpdGpdGpdApdApdApdTpdTpdApdTpdGpdApdTpdTpdCpdCpdTpdGpdTpdTpdGpdGpdTpdCpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp -'3
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
40 mM Tris-HCl, 240 mM NaCl, 10 mM KCl, 2 mM MgCl2, 2 mM CaCl2, and 0.01% Tween-20 in nuclease-free water
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms
Alex M. Yoshikawa, Alexandra Rangel, Trevor Feagin, Elizabeth M. Chun, Leighton Wan, Anping Li, Leonhard Moekl, Michael Eisenstein, Sharon Pitteri, H. Tom Soh
bioRxiv 2021.03.13.435263; doi: https://doi.org/10.1101/2021.03.13.435263
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