hVap1_Stem Aptamer Details
ID# 7922
Structure
Chemistry
DNA
Target
VEGF
Category
Protein
Affinity (Kd)
5.6 nM
Length
48
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dTpdCpdTpdApdGpdCpdCpdCpdApdCpdTpdGpdCpdApdTpdApdGpdApdGpdGpdTpdApdGpdCpdCpdApdCpdGpdTpdApdTpdGpdTpdTpdCp dGpdTpdGpdGpdCpdTpdApdCpdCpdGpdGpdTpdGp
Molecular Weight: 14775.61
Extinction Coefficient: 453400
Binding Conditions / Buffer
APBB; 10 mM HEPES, pH 7.9, 125 mM NaCl, 25 mM KCl, and 1 mM MgCl2
Binding Temp: 23-25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Meng, Hsein-Wei, and John Pagano. "Discovering Aptamers by Cell-SELEX against Human Soluble Growth Factors Ectopically Expressed on Yeast Cell Surface." Plos-One 9.3 (2014): 1-12. Print.
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