HupB-4T Aptamer Details

ID# 7989

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Structure

Chemistry

DNA

Target

HupB

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdGpdTpdApdGpdCpdApdGpdApdTpdGpdGpdGpdGpdGpdGpdGpdGpdGpdGp-3'

Molecular Weight: 7645 g/mole

Extinction Coefficient: 245800

Binding Conditions / Buffer

10 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl

Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14, (2008): 1154-1163. Kalra, P., et al. "G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host Cells." Molecular Therapy: Nucleic Acids. Vol. 13. December 2018. https://doi.org/10.1016/j.omtn.2018.08.011

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