Human Wild-type PAI-1 (WT-15) Aptamer Details
ID# 7619
Structure
Chemistry
2'-F-RNA
Target
Human Wild-type PAI-1
Category
Protein
Affinity (Kd)
177 pM
Length
76
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGprApfUpfCprAprApfCpfUpfCprApfCpfCprGpfUprAprGprGpfUpfCpfUprAprGpfUprGprAprGprAprApfCpfUpfUpfCprAprAprGp fUpfCpfUprApfCpfUpfCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCp-3'
Molecular Weight: 24575.68
Extinction Coefficient: 755600
Binding Conditions / Buffer
Rounds 1-5: BSA Binding Buffer E(20 mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
Rounds 6-7: CHAPS Binding Buffer E( 20 mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.05% CHAPS)
Rounds8-9: CHAPS Binding Buffer F(20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.05% CHAPS)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Blake et al. "Antimetastatic Potential of PAI-1-Specific RNA Aptamers." Oligonucleotides, 19(2009): 117-128.
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