Human Plasma Proteins DNA Aptamer AS1411 Aptamer Details

ID# 8486

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Structure

Chemistry

DNA

Target

Human Plasma Protein

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdTpdGpdGpdTpdGpdGpdTpdGpdGpdTpdTpdGpdTpdGpdGpdTpdGpdGpdTpdGpdGpdTpdGpdGp-3'

Molecular Weight: 8272.3

Extinction Coefficient:

Binding Conditions / Buffer

Binding buffer was composed of DPBS supplemented with 5mM MgCl2, 4.5 g/L glucose (Sangon Biotech), and 1 mg/mL BSA and yeast tRNA (0.1mg/mL, Thermo Fisher Scientific).

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Jia Liu, Zhiqiang Ren, Yang Sun, Liujun Xu, Dali Wei, Weihong Tan, and Ding Ding ACS Nano 2023 17 (23), 24329-24342 DOI: 10.1021/acsnano.3c10238

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