Human Norovirus SMV-25 Aptamer Details

ID# 7933

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Structure

Chemistry

DNA

Target

Human Norovirus

Sequence (3-char table code)

G A C U/T

dApdGpdTpdApdTpdApdCpdGpdTpdApdTpdTpdApdCpdCpdTpdGpdCpdApdGpdCp dCpdApdTpdCpdTpdGpdTpdGpdTpdGpdApdApdGpdApdCpdTpdApdTpdApdTpdGpdGpdCpdGpdCpdTpdCpdApdCpdApdTpdApdTpdTpdTpdCpdTpdTpdTpdCp dCpdGpdApdTpdApdTpdCpdTpdCpdGpdGpdApdGpdApdTpdCpdTpdTpdGpdCp

Molecular Weight: 24864.21

Extinction Coefficient: 770500

Binding Conditions / Buffer

500 ml of binding buffer [consisting of phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20] at Room Temperature

Binding Temp: 24°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Escudero-Abarca BI, Suh SH, Moore MD, Dwivedi HP, Jaykus L-A (2014) Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains. PLoS ONE 9(9): e106805. doi:10.1371/journal.pone.0106805

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