Human Neutrophil Elastase (DNA I) Aptamer Details
ID# 7576
Structure
Chemistry
DNA
Target
Human Neutrophil Elastase (HNE)
Category
Protein
Affinity (Kd)
17 nM
Length
45
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdApdGpdCpdGpdApdTpdApdCpdTpdGpdCpdGpdTpdGpdGpdGpdTpdTpdGpdGpdGpdGpdCpdGpdGpdGpdTpdApdGpdGpdGpdCpdCpdApdGpdCpdApdGpdTpdCpdTpdCpdGpdTp-3'
Molecular Weight: 14046.12
Extinction Coefficient: 428800
Binding Conditions / Buffer
Binding buffer: 150 mM NaCl, 100 mM Tris HCl (pH 7.0), 2 mM MgCl2, 6 mM KCl
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lin et al. "Peptide conjugation to an in vitro-selected DNA ligand improves enzyme inhibition." PNAS, 92(1995): 11044-11048.
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