Human Matrix Metalloprotease 9 (hMMP-9) (F3Bomf) Aptamer Details
ID# 7743
Structure
Chemistry
Chimeric
Target
Human matrix metalloprotease 9 (hMMP-9)
Category
Protein
Affinity (Kd)
20 nM
Length
36
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-fUpmGpfCpfCpfCpfUpmGpfCpfCpfCpfUpfCpmApfCpfCpfCpmGpfUpfUpmApmGpfCpfCpfUpmGpmApmGpfCpmGpfCpfCpfCpfCpmGpfCpmAp-3'
Molecular Weight: 11563.12
Extinction Coefficient: 311900
Binding Conditions / Buffer
SP buffer: 50 mM Tris HCl, pH 7.4, 50 mM NaCl, 100 mM KCl, 5 mM CaCl2, 1 mM Magnesium Acetate.
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
S Gomes et al. A 99mTc-MAG3-aptamer for imaging human tumours associated with high level of matrix metalloproteinase-9. Bioconjugate Chem. (2012). Accepted for print. DOI: 10.1021/bc300146c.
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